Composite

Part:BBa_K1321297:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-20)


sfGFP fused to CBDcex driven by LacI in pSEVA331-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4159
    Illegal suffix found in sequence at 1288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4159
    Illegal SpeI site found at 1289
    Illegal PstI site found at 1303
    Illegal NotI site found at 1296
    Illegal NotI site found at 4165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4159
    Illegal suffix found in sequence at 1289
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4159
    Illegal XbaI site found at 4174
    Illegal SpeI site found at 1289
    Illegal PstI site found at 1303
    Illegal NgoMIV site found at 230
    Illegal NgoMIV site found at 2478
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 245


Design Notes

BBa_K1321297 was created by restricting BBa_K1321357 and BBa_K1321300 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligase products into chemically competent DH10B cells


Source

BBa_K1321357 and BBa_K1321300

References